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1 Digital PCR | NIST
https://www.nist.gov/programs-projects/digital-pcr
Digital PCR (dPCR) is a method used to quantify nucleic acids (DNA, RNA, cDNA). At NIST we are using microfluidic and emulsion-based dPCR ...
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2 Evaluating Digital PCR for the Quantification of Human ...
https://www.nist.gov/publications/evaluating-digital-pcr-quantification-human-nuclear-dna-factors-influencing-target
Evaluating Digital PCR for the Quantification of Human Nuclear DNA: Factors Influencing Target Strandedness ; NIST Pub Series. Special ...
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3 Principles of Digital PCR and Measurement Issues
https://www.nist.gov/document/digital-pcr-101112-v2pdf
Digital PCR Applications and Advances. San Diego, CA ... endorsement by NIST, nor does it imply that ... Quantitative PCR versus Digital PCR.
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4 Evaluating Digital PCR for the Quantification of Human ...
https://www.nist.gov/publications/evaluating-digital-pcr-quantification-human-nuclear-dna-determining-target-strandedness
Digital polymerase chain reaction (dPCR) methodology has been asserted to be a "potentially primary" analytical approach for assigning DNA ...
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5 Evaluating Droplet Digital PCR for the quantification of human ...
https://www.nist.gov/publications/evaluating-droplet-digital-pcr-quantification-human-genomic-dna-lifting-traceability
Digital polymerase chain reaction (dPCR) endpoint platforms directly estimate the number of DNA target copies per reac-tion container, λ, ...
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6 Overview of DNA Programs at NIST
https://www.nist.gov/document/5haynes-dna-quantitaton-digital-pcr-2pdf
Digital PCR and. Quantitation. Ross Haynes. Research Biologist, Applied Genetics Group. Forensics@NIST 2012 Meeting. Gaithersburg, MD.
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7 Evaluating droplet digital PCR for the quantification of human ...
https://www.nist.gov/publications/evaluating-droplet-digital-pcr-quantification-human-genomic-dna-converting-copies
The highly multiplexed polymerase chain reaction (PCR) assays used for forensic human identification perform best when used with an ...
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8 Nanoplate Digital PCR for Wastewater Surveillance
https://www.nist.gov/document/michaelbussman-day2
DHS/NIST Workshop: Standards to Support an Enduring. Capability in Wastewater Surveillance for ... Associate Director, Global Product Management Digital PCR ...
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9 NIST Special Publication 1200-28 - Evaluating Digital PCR for ...
https://nvlpubs.nist.gov/nistpubs/SpecialPublications/NIST.SP.1200-28.pdf
NIST Special Publication 1200-28 ... https://doi.org/10.6028/NIST. ... DNA (dsDNA) can lead digital polymerase chain reaction (dPCR) ...
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10 Evaluating Digital PCR as a primary method for the ...
https://www.nist.gov/publications/evaluating-digital-pcr-primary-method-quantification-human-genomic-dna-accessible
Polymerase chain reaction (PCR) assays perform best when the input quantity of template DNA is controlled to within about a factor of √2.
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11 Exploring Digital PCR as a primary method for the ...
https://www.nist.gov/publications/exploring-digital-pcr-primary-method-quantification-human-genomic-dna-ogive-plots-and
Polymerase chain reaction (PCR) end-point limiting dilution techniques, collectively termed "digital PCR (dPCR)", have been proposed as a ...
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12 NIST Special Publication 1200 27: Evaluation of Methods for ...
https://www.nist.gov/publications/nist-special-publication-1200-27-evaluation-methods-assessing-proportion-single
This report documents our evaluation of three digital PCR (dPCR) based methods for estimating the proportion, if any, of single-stranded DNA ...
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13 SARS-CoV-2 Research Grade Test Material | NIST
https://www.nist.gov/programs-projects/sars-cov-2-research-grade-test-material
We have characterized this material by size and multiple reverse transcription droplet digital PCR (RT-ddPCR) assays as well as qPCR methods. A ...
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14 Guidelines for Minimum Information for Publication of ...
https://www.nist.gov/publications/guidelines-minimum-information-publication-quantitative-digital-pcr-experiments
There is growing interest in the digital polymerase chain reaction (dPCR) as technological progress makes it a practical and increasingly ...
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15 COVID-19 Detection Dr. Pete Vallone
https://www.nist.gov/document/covid-19-detection-0
PCR-Based DNA Profiling Standard (2391d) ... multiplex PCR, real-time PCR, and digital ... Controls and Standards: What would a NIST.
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16 Evaluating Digital PCR for the Quantification of Human ...
https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=917985
NIST-Developed Assays. Eight PCR assays developed at. NIST were used for this study. Five of these assays target nucleotide sequences related to ...
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17 Steps to Achieve Quantitative Measurements of microRNA ...
https://www.nist.gov/publications/steps-achieve-quantitative-measurements-microrna-using-two-step-droplet-digital-pcr
Steps to Achieve Quantitative Measurements of microRNA Using Two Step Droplet Digital PCR. Published. November 16, 2017 ...
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18 RNA Reference Materials Are Useful for Standardizing COVID ...
https://www.nist.gov/news-events/news/2022/01/rna-reference-materials-are-useful-standardizing-covid-19-tests-study-shows
NIST researcher Megan Cleveland uses a PCR machine to amplify DNA ... measured and validated the reference materials using digital PCR.
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19 Digital PCR for the Characterization of Reference Materials
https://www.bipm.org/documents/20126/53312137/17-MCleveland_JCTLM_2019.pdf/7908de89-7c1b-99ce-df2d-dbc9399541b1
Digital PCR at NIST. Digital PCR has become our 'go to' method for the quantification of nucleic acid-based materials. Replacing UV spectroscopy (indirect ...
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20 Development and interlaboratory evaluation of a NIST ... - NCBI
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875440/
The reference values for the ratios of the EGFR and MET gene copy numbers to the copy numbers of reference genes were measured using digital PCR ...
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21 Digital MIQE Guidelines Update: Minimum Information for ...
https://academic.oup.com/clinchem/article/66/8/1012/5880117
Abstract. Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR ...
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22 RNA reference materials with defined viral RNA loads ... - PLOS
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262656
... by RT-qPCR using synthetic SARS-CoV-2 RNA standards and digital PCR. ... UK), the National Institute of Standards and Technology (NIST, ...
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23 Utilization of Digital PCR in Quantity Verification of Plasmid ...
https://www.frontiersin.org/articles/10.3389/fmolb.2020.00155/full
In this study, we utilized digital PCR (dPCR) for absolute ... Production and certification of NIST standard reference material 2372 human ...
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24 Digital PCR inhibition mechanisms using standardized ...
http://liu.diva-portal.org/smash/record.jsf?pid=diva2:1047193
National Institute of Standards and Technology (NIST). ... Digital PCR (dPCR) enables absolute quantification of nucleic acids by ...
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25 Specifications QIAcuity.docx - AWS
https://imlive.s3.amazonaws.com/Federal%20Government/ID216255577669210152869852586835199112158/Specifications%20QIAcuity.docx
Digital polymerase chain reaction (PCR) is used to determine the accurate and reproducible concentration of DNA samples. NIST requires a Digital PCR ...
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26 Genomic DNA standards for HER2 measurements
https://www.sciencedirect.com/science/article/pii/S2214753516300031
Development of NIST standard reference material 2373: Genomic DNA standards for HER2 ... Garcia-Murillas used dPCR to measure the gene copy ratio of HER2 to ...
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27 Production and certification of NIST Standard Reference ...
https://europepmc.org/article/med/19377837
Quantification of plasmid DNA standards for U.S. EPA fecal indicator bacteria qPCR methods by droplet digital PCR analysis.
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28 (PDF) Comparison of Droplet Digital PCR to Real-Time PCR ...
https://www.researchgate.net/publication/233886666_Comparison_of_Droplet_Digital_PCR_to_Real-Time_PCR_for_Quantitative_Detection_of_Cytomegalovirus
Ten-fold serial dilutions of the World Health Organization (WHO) and the National Institute of Standards and Technology (NIST) CMV quantitative standards were ...
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29 NIST Creating Reference Material for SARS-CoV-2 Diagnostic ...
https://www.genomeweb.com/tools-technology/nist-creating-reference-material-sars-cov-2-diagnostic-assay-developers
NIST characterized this material by multiple reverse transcription droplet digital PCR (RT-ddPCR) assays as well as qPCR methods.
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30 Counting DNA Molecule by Molecule - Bio-Rad
https://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_7374.pdf
In support of the use of Droplet Digital PCR for reference material validation, the National Institute of Standards and. Technology (NIST) in the U.S. has ...
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31 x00AE; 2917 - City of Santa Cruz
https://www.cityofsantacruz.com/home/showpublisheddocument/90861/638001249517761153
terial® 2917 (NIST SRM® 2917) is a DNA plasmid construct that functions with ... Dx real-time PCR system (Thermo Fisher Scientific).
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32 Combinati Absolute Q Digital PCR NB645060-21-01899 - GovTribe
https://govtribe.com/opportunity/federal-contract-opportunity/combinati-absolute-q-digital-pcr-nb6450602101899
The National Institute of Standards and Technology (NIST) intends to negotiate on a sole source basis, under the authority of FAR 13.5, Combinati Incorporated, ...
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33 Clinical Genetics - Opportunity
https://nrc58.nas.edu/RAPLab10/Opportunity/Opportunity.aspx?LabCode=50&ROPCD=506451&RONum=B8263
Opportunity at National Institute of Standards and Technology (NIST) ... include copy number determination by quantitative PCR and digital PCR, ...
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34 Digital PCR Applications in the SARS-CoV-2/COVID-19 Era
https://journals.asm.org/doi/abs/10.1128/cmr.00168-21?af=R
In contrast, digital PCR (dPCR), the third generation of PCR, has been shown to be more effective than the gold standard, RT-qPCR, ...
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35 Comparison of Droplet Digital PCR to Real-Time PCR for ...
http://gmo-qpcr-analysis.com/hayden-et-al-dpcr-2013.pdf
Real-time PCR showed somewhat higher sensitivity than ddPCR (LODs, 3 log10 versus 4 log10 cop- ies/ml and IU/ml for NIST and WHO standards, respectively).
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36 What is Digital PCR? - AZoLifeSciences
https://www.azolifesciences.com/article/What-is-Digital-PCR.aspx
The primary difference between qPCR and digital PCR (dPCR) is that with dPCR, the sample is diluted into reaction chambers known as partitions, ...
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37 Quantification of BK Virus Standards by Quantitative Real ...
https://search.proquest.com/openview/f8b529e34c5119b9f83569b7706ad165/1?pq-origsite=gscholar&cbl=47786
Two preparations of NIST BKV clones were also tested. ... We evaluated all materials with both real-time PCR and droplet digital PCR (ddPCR) assays to more ...
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38 NIST Development of a SARS-CoV-2 Test Material
https://static1.squarespace.com/static/5739222a27d4bd28d98e3ce9/t/5f11f52f699c3b522865d52b/1595012426980/20200717_Vallone_NIST_Standards_Slides.pdf
multiplex PCR, real-time PCR, and digital. PCR are used to genotype, sequence, and provide quantitative information.
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39 Limitations of methods for measuring the concentration of ...
https://www.implen.de/wp-content/uploads/docs/Limitations-of-methods-for-measuring-the-concentration-of-human-genomic-DNA-and-oligonucleotide-samples.pdf
Quantification of DNA using droplet dPCR. The sequences of the primers and probes for the digital PCR assays developed at NIST for.
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40 Certification of Standard Reference Material 2365 BK Virus ...
https://www.govinfo.gov/content/pkg/GOVPUB-C13-78e896db61052555906e15af865f63a1/pdf/GOVPUB-C13-78e896db61052555906e15af865f63a1.pdf
Walter Copan, NIST Director and Under Secretary of Commerce for Standards and ... preparing the BK construct and developing digital PCR assays used in the ...
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41 Accurate Bulk Quantitation of Droplet Digital Polymerase ...
https://pubs.acs.org/doi/10.1021/acs.analchem.1c00877
Droplet digital PCR provides superior accuracy for nucleic acid quantitation. The requirement of microfluidics to generate and analyze the ...
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42 Genomic DNA standards for HER2 measurements
https://www.semanticscholar.org/paper/Development-of-NIST-standard-reference-material-DNA-He-Almeida/82a75989d6aee5c3f2dea072ef7167dfdbd4cacf
Development of NIST standard reference material 2373: Genomic DNA ... Multiplex Droplet Digital PCR Assay for Detection of MET and HER2 ...
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43 A Plasmid DNA Standard for Molecular Recreational ... - EPA
https://cfpub.epa.gov/si/si_public_record_Report.cfm?dirEntryId=352195&Lab=CEMM
DHS/NIST Standards to Support an Enduring Capability in Wastewater ... The material was characterized by NIST using droplet digital PCR to ...
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44 Measuring the Sensitivity of COVID Tests With New Material From ...
https://www.ishinews.com/measuring-the-sensitivity-of-covid-tests-with-new-material-from-nist/
NIST measured this concentration multiple times using a technique called digital PCR, or dPCR, which counts the number of individual DNA fragments in a ...
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45 NIST PCR-BASED DNA PROFILING STANDARD (SRM 2391D)
https://vb6ykw2twb15uf9341ls5n11-wpengine.netdna-ssl.com/wp-content/uploads/2019/08/48-Carolyn-Steffen.pdf
NIST PCR-BASED DNA PROFILING STANDARD (SRM 2391D): WHAT IS NEW AND ... concentrations were determined by droplet digital PCR (ddPCR) and are listed with an.
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46 Certified DNA Reference Materials to Compare HER2 Gene ...
https://www.jmdjournal.org/article/S1525-1578(16)30091-5/fulltext
qPCR and dPCR at NIST ; EIF5-P · 5′-TTCAGCCTTCTCTTCTCATGCAGTTGTCAG-3′, FAM ; 2PR4-P · 5′-TTTGTCTACCACTTGCAAAGCTGGCCTTT-3′, FAM ; R4Q5-P · 5′- ...
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47 Development of SARS-CoV-2 packaged RNA reference ...
https://link.springer.com/article/10.1007/s00216-021-03846-y
To measure the homogeneity and stability of the lentiviral SARS-CoV-2 RM, reverse transcription droplet digital PCR (RT-ddPCR) was used with in- ...
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48 NIST develops genetic material for validating ... - Medical Xpress
https://medicalxpress.com/pdf578144845.pdf
measured the concentration of DNA in the material using a high-precision technique called digital PCR. And they worked with NIST statisticians to ensure.
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49 NIST Develops Genetic Material for Validating Monkeypox Tests
https://www.hstoday.us/subject-matter-areas/pandemic-biohazard/nist-develops-genetic-material-for-validating-monkeypox-tests/
They measured the concentration of DNA in the material using a high-precision technique called digital PCR. And they worked with NIST ...
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50 Digital PCR Technology Report: An Insight to Vendors, Costs ...
https://www.insightpharmareports.com/ProductTab/ProductCustomTab/897
5.3 What is the Process of the RainDrop Digital PCR System? ... Chapter 12: National Institute of Standards and Technology (NIST), United States.
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51 Development and usage of a NIST standard reference ...
https://www.fsigeneticssup.com/article/S1875-1768(08)00243-6/abstract
Developmental validation of a real-time quantitative PCR assay for automated quantification of human DNA. J. Forensic Sci. 2003; 48: 1041-1046. View ...
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52 Multiplex Droplet Digital PCR Assay for Detection of MET and ...
https://www.mdpi.com/2072-6694/14/6/1458/htm
We developed the first multiplex droplet digital PCR assay for the ... Development and interlaboratory evaluation of a NIST Reference Material RM 8366 for ...
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53 Evaluating digital PCR for the quantification of human nuclear ...
https://ouci.dntb.gov.ua/en/works/4wwGRRm4/
Romsos EL, Kline MC, Duewer DL, Toman B, Farkas N. Certification of standard reference Material® 2372a human DNA quantitation standard. NIST Special Publication ...
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54 Digital PCR - 2017 Next Generation Dx Summit
https://www.nextgenerationdx.com/digital-pcr/17
Digital PCR remains an important technology to have in diagnostic labs, ... NIST is developing genomic reference material standards for gene copy ...
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55 Applied Biosystems PCR thermal cyclers and plastics
https://tools.thermofisher.com/content/sfs/brochures/applied-biosystems-pcr-thermal-cyclers-plastics-brochure.pdf
the QuantStudio™ 3D Digital PCR System. Ultimate flexibility and throughput. ProFlex PCR System. Find out more at thermofisher.com/proflex. 3 x 32-well.
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56 dPCR – the digital polymerase chain reaction
https://www.rsc.org/images/TB_79_tcm18-249823.pdf
from NIST);. Viral load determination (e.g. level of virus in 1 mL of blood). Relative quantitation. Copy Number Variation (CNV);. Relative ...
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57 Critical assessment of digital PCR for the detection and ...
https://d-nb.info/1160178844/34
However, digital PCR (dPCR) offers several advantages over qPCR, making this new technique ... NIST special publication 260-184.
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58 Comparison of Droplet Digital PCR to Real ... - Emory University
https://open.library.emory.edu/publications/emory:s2bh1/pdf/
Real-time PCR showed somewhat higher sensitivity than ddPCR (LODs, 3 log10 versus 4 log10 cop- ies/ml and IU/ml for NIST and WHO standards, ...
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59 Quantitative Analysis with Droplet Digital PCR
https://experiments.springernature.com/articles/10.1007/978-1-4939-8837-2_14
Digital PCR-based methods, such as droplet digital PCR, are one of the best tools for determination of ... Accessed 14 Dec 2017https://doi.org/10.6028/NIST.
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60 Limitations of methods for measuring the ... - Future Science
https://www.future-science.com/doi/10.2144/btn-2017-0102
Quantification of DNA using droplet dPCR. The sequences of the primers and probes for the digital PCR assays developed at NIST for measuring ...
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61 deprez-et-al-digital-pcr-2016.pdf - Gene-Quantification.info
https://www.gene-quantification.de/deprez-et-al-digital-pcr-2016.pdf
Validation of a digital PCR method for quantification of DNA copy number concentrations by using a certified reference material.
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62 Steps to achieve quantitative measurements of ... - Data.gov
https://catalog.data.gov/dataset/steps-to-achieve-quantitative-measurements-of-microrna-using-two-step-droplet-digital-pcr-41d37
Droplet digital PCR (ddPCR) is being advocated as a reference method to measure rare genomic targets. It has consistently been proven to be more sensitive ...
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63 NIST Develops Genetic Material for Validating Monkeypox Tests ...
https://mid.nmdc.ac.cn/index.php/node/1037016
NIST is making the material, which contains gene fragments from the virus ... of DNA in the material using a high-precision technique called digital PCR.
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64 department - ATCC
https://www.atcc.org/~/media/781B27D7A4A34C6EAFF669663C24CD25.ashx
Maintenance of SRM Certification: NIST will monitor this SRM over the period ... For use with digital PCR polymerase chain reaction (dPCR) Assays: Use the ...
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65 Evaluating digital PCR for the quantification of human ... - Gale
https://go.gale.com/ps/i.do?id=GALE%7CA628319365&sid=googleScholar&v=2.1&it=r&linkaccess=abs&issn=16182642&p=AONE&sw=w
We and our colleagues at the National Institute of Standards and Technology (NIST) have explored whether and how digital polymerase chain reaction (dPCR) ...
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66 Quantification of plasmid DNA reference materials for Shiga ...
https://bmcchem.biomedcentral.com/articles/10.1186/s13065-016-0201-0
Digital PCR distributed the DNA samples onto a microarray chip containing ... Institute of Standards and Technology (NIST) reported that, ...
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67 An Introduction to Forensic DNA Typing
https://www.aps.org/units/maspg/meetings/upload/vallone-06-16-21.pdf
How work at NIST supports the Forensic DNA Typing community ... Estimation of Extraction Efficiency by Droplet Digital PCR.
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68 RNA Reference Materials Are Useful for ... - Mirage News
https://www.miragenews.com/rna-reference-materials-are-useful-for-710350/
NIST researcher Megan Cleveland uses a PCR machine to amplify DNA ... measured and validated the reference materials using digital PCR.
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69 A multiplexed droplet digital PCR assay performs better than ...
http://gene-quantification.net/sedlak-et-al-dpcr-qpcr-2014.pdf
The assay was validated using CMV NIST DNA (Haynes et al., 2013), AdVs species F (types 40 and 41) ATCC DNA, and EXO plasmid DNA spiked individually and as.
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70 AstraZeneca R&D arm signs five-year deal with NIST to ...
https://www.outsourcing-pharma.com/Article/2015/02/23/AstraZeneca-R-D-arm-signs-five-year-deal-with-NIST-to-advance-discovery-manufacturing
Work will be conducted at both the MedImmune and NIST campuses, which are located near each other in Gaithersburg, MD. ... dPCR Case Study.
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71 NIST Standard Reference Materials Catalogue - Sigma-Aldrich
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/231/138/nist-standard-reference-materials-catalogue-rtp.pdf
The National Institute of Standards and Technology (NIST) is one ... 1957 – The first digital image. 1956 – The fall of parity. 1954 – NIST Boulder labs ...
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72 The Digital MIQE Guidelines Update - mediaTUM
https://mediatum.ub.tum.de/doc/1575228/file.pdf
stop, however, with the term 'digital PCR' being coined by Vogelstein and Kinzler in 1999 (7) as a ... of a NIST reference material RM 8366 for EGFR and.
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73 Digital Thermometer, Type K/J, NIST Traceable Calibration
https://www.southernlabware.com/digital-thermometer-type-k-j-nist-traceable-calibration.html
Digital Thermometer, Type K/J, NIST Traceable Calibration from Digi-Sense at Southern Labware. Checkout our huge collection of laboratory supplies, ...
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74 SeraCare and NIST Partner on Development of Circulating Tumor ...
https://www.diagnosticsworldnews.com/news/2016/07/14/seracare-and-nist-partner-on-development-of-circulating-tumor-dna-reference-standards-for-diagnostics
SeraCare and NIST Partner on Development of Circulating Tumor DNA Reference ... NIST for development of digital PCR measurement methodologies, and NIST will ...
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75 Accurate Digital Polymerase Chain Reaction Quantification of ...
https://research.chalmers.se/publication/248764
Digital PCR (dPCR) enables absolute quantification of nucleic acids by partitioning of the sample ... National Institute of Standards and Technology (NIST) ...
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76 Single-day HER2neu amplification assessment using chip ...
https://www.tandfonline.com/doi/full/10.2147/BCTT.S161264
The present study was designed to evaluate a chip-based digital PCR (dPCR) system for assessing HER2 amplification from formalin-fixed paraffin- ...
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77 Droplet volume variability as a critical factor for accuracy of ...
https://www.academia.edu/34603444/Droplet_volume_variability_as_a_critical_factor_for_accuracy_of_absolute_quantification_using_droplet_digital_PCR
AutoDG™ Droplet Digital™ PCR system. And although In dPCR, the copy number concentration in the sample is the same droplet volume was pre-set regardless of the ...
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78 InDel Analysis - COBO Technologies
https://cobotechnologies.com/tags/indel-analysis/
Digital droplet PCR and IDAA for the detection of... 29-04-2020 ... COBO Technologies will attend NIST Genome Editing... 26-04-2019.
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79 PCR solutions brochure - Fisher Scientific
https://beta-static.fishersci.com/content/dam/fishersci/en_US/documents/programs/scientific/brochures-and-catalogs/brochures/fisher-scientific-pcr-solutions-brochure.pdf
20K Chip is compatible with the QuantStudio 3D Digital PCR System. ... to standards traceable to the National Institute of Standards and Technology (NIST).
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80 Need Better Standards for Your Clinical Assays? NIST Can Help ...
https://mendelspod.com/podcasts/need-better-standards-your-clinical-assays-nist-can-help/
13:42 Digital PCR proving powerful. 21:06 Partnering with the community. The life science tools space is flourishing. Biomedical research output is at an ...
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81 NIST Research & Forensic Science
https://www.justice.gov/file/929216/download
NIST Focus Area: Digital Forensics. Objectives: To provide standards and measurement to improve the quality, efficiency and understanding of digitally-based ...
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82 SeraCare, NIST sign 3-year CRAD agreement to advance ...
http://test.pharmabiz.com/news/seracare-nist-sign-3-year-crad-agreement-to-advance-circulating-tumour-dna-reference-standards-for-diagnostics-96292
SeraCare, NIST sign 3-year CRAD agreement to advance circulating tumour DNA reference ... to NIST for development of digital PCR measurement methodologies, ...
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83 Inter-laboratory assessment of different digital PCR platforms ...
https://cyberleninka.org/article/n/110482
Comparison of four digital PCR platforms for accurate quantification of DNA copy ... Science, University of Surrey, Guildford, Surrey GU2 7XH, UK NIST ...
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84 NIST Develops Genetic Material for Validating Monkeypox Tests ...
https://commercenewswire.com/stories/629503941-nist-develops-genetic-material-for-validating-monkeypox-tests
NIST is making the material, which contains gene fragments from the virus ... of DNA in the material using a high-precision technique called digital PCR.
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85 NIST Sigma Xi Early-Career Poster Presentation (ECPP)
https://www.sigmaxiatnist.org/ecpp
NIST Sigma Xi Early-Career Poster Presentation (ECPP) ... Erica Stein, "Considerations for digital droplet PCR to quantitatively measure miRNA using a ...
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86 SeraCare and NIST Partner on Development of Circulating ...
https://www.prnewswire.com/news-releases/seracare-and-nist-partner-on-development-of-circulating-tumor-dna-reference-standards-for-diagnostics-300299009.html
SeraCare and NIST Partner on Development of Circulating Tumor DNA ... to NIST for development of digital PCR measurement methodologies, ...
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87 Digital PCR | IDT - Integrated DNA Technologies
https://www.idtdna.com/pages/technology/qpcr-and-pcr/digital-pcr
Overview. Digital PCR (dPCR) provides a highly sensitive measure of the absolute amount of a target DNA. Because of ...
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88 Digital PCR | Nanoplate dPCR technology - QIAGEN
https://www.qiagen.com/us/applications/digital-pcr
Discover a fully integrated microfluidic nanoplate digital PCR technology for absolute quantification using a qPCR-like workflow and scalable products.
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89 Specifications Digital PCR Final.pdf Contract Doc | HigherGov
https://www.highergov.com/document/specifications-digital-pcr-final-pdf-0f7b18/
View and Download Contract Opportunity File Specifications Digital PCR Final.pdf Uploaded On 1/13/22.
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90 S4 Table
https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0262656/1/pone.0262656.s006.docx?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20221126%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20221126T020428Z&X-Goog-Expires=86400&X-Goog-SignedHeaders=host&X-Goog-Signature=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
Measurement results determined by digital PCR by NML, NIST and PTB for RM 1 and RM 2. ... NIST, CDC N2, 1, 1, 12030189.
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91 Medical Device News and Press Releases|Japan MDC, LLC
https://www.j-mdc.com/en/news/index.php
ClariPi Joins Siemens Healthineers Digital Marketplace ... Office Lab Implementation and Consulting Services of PCR Technologies for UroGPO Members.
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92 The Grimerica Show
https://grimerica.ca/category/thegrimericashow/
... digital consciousness · digital currency · digital simulation · digital universe ... night terrors · nightmare · Nightmares · Nile · NIST · NIST Report ...
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93 Issues in Life Sciences—Muscle, Membrane, and General ...
https://books.google.com/books?id=M97KpBjLDTUC&pg=PT13&lpg=PT13&dq=nist+digital+pcr&source=bl&ots=BCWfVfYE3O&sig=ACfU3U0OGFIHh19stATCkbA1vhhyKTvbLA&hl=en&sa=X&ved=2ahUKEwir_aKlx9b7AhXVh1YBHbiCA7IQ6AF6BQjiAhAD
Digital PCR offers highly precise, direct quantification without requiring a ... of Standards and Technology (NIST) CMV quantitative standards were tested, ...
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94 Evaluation Technologies for Food Quality
https://books.google.com/books?id=pL6SDwAAQBAJ&pg=PA650&lpg=PA650&dq=nist+digital+pcr&source=bl&ots=D-DGxE7qR5&sig=ACfU3U37yi-79lAgxxVT2USVKi1dKEMbUA&hl=en&sa=X&ved=2ahUKEwir_aKlx9b7AhXVh1YBHbiCA7IQ6AF6BQjjAhAD
methods for DNA quantification: UV, HR-ICP-MS, and dPCR, each of which ... 25.7C) by using a phosphorus concentration certified RM (SRM3139a from NIST).
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