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1 Lysozyme (L7651) - Datasheet - Sigma-Aldrich
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/362/900/l7651dat.pdf
Lysozyme from chicken egg white for Molecular Biology ... Lysozyme is a single chain polypeptide of 129 amino ... Isoelectric point (pI):11 11.35.
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2 Purification and Characterization of Recombinant Human ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694923/
The molecular weight of lysozyme is approximately 14 kDa to 15 kDa and the isoelectric point is about pH 11 [33].
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3 Lysozyme - Wikipedia
https://en.wikipedia.org/wiki/Lysozyme
The isoelectric point of human milk lysozyme is 10.5–11.
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4 Values of the pH at the isoelectric point, pI for lysozyme ...
https://www.researchgate.net/figure/Values-of-the-pH-at-the-isoelectric-point-pI-for-lysozyme-fragments-not-included-in_tbl2_23628148
... pI isoelectric points (calculated and experimental) for lysozyme and its fragments not included in the fit are reported in Table 4. The calculation result ...
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5 Isolation of Lysozyme from Hen Egg Albumen by Alcohol ...
https://people.uleth.ca/~steyqj/Bchm3300Lab/Paper_2.pdf
water and its pH value was adjusted to 8.0 before being subjected to alcohol-insoluble ... an isoelectric point between 10.5 to 11.5 and is stable under.
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6 3.2.1.17: lysozyme - BRENDA Enzyme Database
https://www.brenda-enzymes.org/all_enzymes.php?ecno=3.2.1.17&table=pI_Value
3.2.1.17: lysozyme · pI Value on EC 3.2.1.17 - lysozyme.
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7 Lysozyme - Worthington Enzyme Manual
https://www.worthington-biochem.com/products/lysozyme/manual
Lysozyme - Manual ; Protein Accession Number. P00698 ; CATH Classification. Class: Mainly Alpha; Architecture: Orthogonal Bundle; Topology: Lysozyme ; Molecular ...
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8 isolation of hen egg white lysozyme by cation exchange ...
https://innovareacademics.in/journals/index.php/ajpcr/article/download/11462/5095
Differences between mean values were determined by the analysis of variance (ANOVA). ... Hen egg white lysozyme is a protein with high isoelectric point.
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9 Solved The protein lysozyme has an isoelectric point of pI
https://www.chegg.com/homework-help/questions-and-answers/protein-lysozyme-isoelectric-point-pi-1100-ph-number-positive-charges-protein-surface-equa-q82663042
Question: The protein lysozyme has an isoelectric point of pI = 11.00. This is the pH at which the number of positive charges on the protein surface equals the ...
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10 Antibacterial Activity of Hen Egg White Lysozyme ... - Scinapse
https://asset-pdf.scinapse.io/prod/2898869528/2898869528.pdf
egg white lysozyme (HEWL) with thermal and chemical treatments against Gram-negative bacteria. ... with a pI value of 10.7 [2].
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11 Batch purification of high-purity lysozyme from egg white and ...
https://www.tandfonline.com/doi/full/10.3109/13880200903196859
According to the isoelectric point (pI) of lysozyme (pI = 11), five different Amberlite cation ... Values are the means from three independent experiments.
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12 Sequential Separation of Lysozyme ... - Iowa State University
https://dr.lib.iastate.edu/bitstreams/fef3977c-fb94-436f-a49d-eefaa2e5dc56/download
lysozyme. The most frequent separation technique used for lysozyme is based on cation exchange chromatography mainly due to its high pI value.
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13 Small Angle Neutron Scattering studies of Lysozyme solutions
https://www.nist.gov/system/files/documents/2021/01/19/SANS2_Protein_Solutions-v5.pdf
Generally, both c- and g-lysozymes are basic proteins due to their high isoelectric point (pI) values. c-Lysozyme is a single chain of 129 ...
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14 Acrididae) | Journal of Insect Science | Oxford Academic
https://academic.oup.com/jinsectscience/article/15/1/57/2583151
Some insect lysozymes also exhibits antifungal activity by ... This basic pI value of Sg l (>9) is comparable to pI of lysozymes of H.
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15 US4552845A - Method for separating lysozyme from egg-white
https://patents.google.com/patent/US4552845A/en
The eluted lysozyme and the separated albumin fraction are further ... of pI 10.5, molecular weight approximately 14,000 Daltons, and S20, W value of 1.9.
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16 Structure, thermostability, and conformational flexibility of hen ...
https://www.pnas.org/doi/pdf/10.1073/pnas.96.4.1262
and representative hydrophilic protein hen egg-white lysozyme. (pI 11) (3), lyophilized from pH 6, exceeds 10 mg/ml in more than a dozen nonaqueous solvents ...
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17 Effective and Low-cost Purification of Lysozyme by ...
http://maxwellsci.com/msproof.php?doi=ajfst.6.64
Keywords: Gel chromatography, isoelectric point precipitation, lysozyme, ... pI values and only lysozyme (Mr 14,300) and avid in.
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18 Strategies to Recover and Purify Lysozyme From Egg White
https://iontosorb.cz/pages/mag-clanky/5_Chapter_Lysozyme.pdf
Some physicochemical properties of hen egg white lysozyme. Property. Value. Molecular weight Nº of subunits. 14,400 1. Amino acids. 129. pI Disulfide bonds.
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19 · Lysozyme Net Charge and Ion Bind~g in Concentrated ...
https://www.osti.gov/servlets/purl/1183892
of lysozyme, on pKa values of the various titratable groups, ... point, p0 l, is given by p0 l = pi + logy H+ and was assumed not to vary with ionic ...
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20 Sequential separation of lysozyme, ovomucin ... - CiteSeerX
https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=0c8fced9d861a25aa237f2f259cb79b0cff4576a
ration technique used for lysozyme is based on cation exchange chromatography mainly due to its high pI value. Carboxymethyl cellulose (CMC) was used in.
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21 BC 367 Experiment 3 Purification and Characterization of the ...
https://www.colby.edu/chemistry/CH367/laboratory/expt3.pdf
characteristics of lysozyme, which has an unusually high pI of 10.5, ... Assume that the absorbance value for a particular fraction corresponds to a protein.
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22 0.1 mg/mL Lysozyme | Entegris
https://www.entegris.com/content/dam/product-assets/nicompnanodlszlssystems/technote-01-mg-ml-lysozyme-10566.pdf
Note: This small PI value is expected for a well prepared sample with count rate ~160 kcps. The 1 mg/mL lysozyme sample was placed into a clean round glass ...
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23 Lysozyme - Thermo Fisher Scientific
https://www.thermofisher.com/order/catalog/product/89833
Thermo Scientific Lysozyme is an enzyme characterized by the ability to break down the bacterial cell wall to improve protein or nucleic acid extraction ...
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24 Separation of proteins with cation exchange chromatography ...
https://www.knauer.net/Application/application_notes/VBS0072_Separation%20of%20proteins%20with%20cation%20exchange%20chromatography%20on%20sepapure%20SM%20and%20CM_Final%20-web-.pdf
At a pH below their pI proteins ... Cytochrome C (pI 10.3), Lysozyme (pI 11.35), and Ribo- ... values, which make them ideal candidates for cation.
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25 Application to Lysozyme - sciforum
https://sciforum.net/manuscripts/1370/original.pdf
amino acids can be used to estimate the isoelectric point of lysozyme and its ... Values of the Gk and Jk charge-transfer indices up to fifth order for 21 ...
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26 lysozyme v5 - Universidad de Zaragoza
https://zaguan.unizar.es/record/30717/files/TAZ-TFG-2015-011.pdf
polyethilenglycol and ion exchange chromatography, obtaining lysozyme of great purity and ... CEW proteins and their isoelectric point (pI) values.
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27 1LPI: HEW LYSOZYME: TRP...NA CATION-PI INTERACTION
https://www.rcsb.org/structure/1LPI
Method: X-RAY DIFFRACTION; Resolution: 2.00 Å; R-Value Free: 0.269 ... Cation-pi (Na+-Trp) interactions in the crystal structure of tetragonal lysozyme.
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28 Adsorption of Lysozyme Into a Charged Confining Pore - bioRxiv
https://www.biorxiv.org/content/10.1101/2021.07.11.451934v1.full.pdf
solution pH gets closer to the HEWL isoelectric point. ... To set the value of εc, we associate the unfolding–transition.
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29 Antibacterial Activity of Hen Egg White Lysozyme Denatured ...
https://www.mdpi.com/2218-0532/86/4/48/htm
HEWL has a high isoelectric point (pI) with a pI value of 10.7 [2]. Hen's egg is the richest HEWL source, accounting for 3.5% of total egg white proteins ...
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30 Cloning, purification and comparative characterization of two ...
https://www.scielo.br/j/bjmbr/a/pCdtC8jZRjZdHPbzyBWnbKf/?lang=en&format=pdf
more acidic value, pH 3.8, at a high ionic strength (µ = 0.2). However, the pH optimum of their ... egg white lysozyme (HEWL) is the most extensively stud-.
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31 Characterization of protein adsorption onto silica nanoparticles
https://link.springer.com/article/10.1007/s00396-015-3754-x
For lysozyme (pI ≈ 11), the adsorption level increases with pH and reaches a value corresponding to about two close-packed monolayers at pH ...
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32 Ion Exchange Chromatography
https://research.fredhutch.org/content/dam/stripe/hahn/methods/biochem/Ion_Exchange_Chromatography_Handbook.pdf
pI values migrate at different rates as the pH gradient develops, continually binding and ... Mixture of chymotypsinogen, cytochrome C, and lysozyme.
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33 electrophoresis - Molecular Weight Determination by SDS-PAGE
https://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_3133.pdf
on the values obtained for the bands in the MW standard. The MW of the unknown protein band is ... (for example, lysozyme, histones, and troponin I), which.
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34 A one-step purification of a plant lysozyme from in vitro ...
https://core.ac.uk/download/pdf/82724061.pdf
filtered growth medium was used as a lysozyme source The enzyme was purified by a rapid one-step punfi- ... Published data [4-6,13,14] indicated a pi value.
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35 Electrophoresis - protein - Britannica
https://www.britannica.com/science/protein/Electrophoresis
The isoelectric point, the pH value at which the protein molecule does not migrate, is in the range of pH 5 to 7 for many proteins.
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36 Molecular Cloning and Characterization of a New C-type ...
https://www.animbiosci.org/m/makeCookie.php?url=/m/journal/view.php?number=23282
Milk lysozyme is the ubiquitous enzyme in milk of mammals. ... and the pI value in the case of yak stomach lysozyme was 10.58, it was necessary to determine ...
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37 Lysozyme - EliScholar
https://elischolar.library.yale.edu/cgi/viewcontent.cgi?article=3018&context=ymtdl
Since lysozyme has an isoelectric point of 10.5, there are not many naturally occuring compounds that have a higher isoelectric point.
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38 LYZ - Lysozyme C - Homo sapiens (Human) | UniProtKB
https://www.uniprot.org/uniprotkb/P61626/entry
Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and ...
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39 Extraction and Characterization of Lysozyme from Salted Duck ...
https://mdpi-res.com/d_attachment/foods/foods-11-03567/article_deploy/foods-11-03567.pdf?version=1667992667
study creatively used isoelectric point precipitation, ... experiments were conducted to take the average value and draw the standard curve.
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40 properties and application of egg white lysozyme and its ...
http://journal.pan.olsztyn.pl/pdf-98099-30714?filename=PROPERTIES%20AND.pdf
Lysozyme (E.C.3.2.17, N-acetyl-muramic-hydrolase) is a bacteriolytic enzyme commonly found in nature. It is a globular basic protein characterised by MW of ...
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41 Structure Determination of Hen Egg-White Lysozyme ...
https://pubs.acs.org/doi/10.1021/acs.langmuir.9b03826
The monolayer reaches a stable surface pressure value after ∼30 min. ... Lysozyme's pI is ∼10.5, and thus, at pD = 4.5, it bears a net ...
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42 Low Concentration Protein Lysozyme Measurements - AZoNano
https://www.azonano.com/article.aspx?ArticleID=4110
It is to be noted that the PI value is small as anticipated for a well- prepared sample with count rate ~160 kcps. The 1mg/mL lysozyme ...
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43 How buffer pH and NaCl affect size exclusion chromatography
https://www.cytivalifesciences.com/solutions/protein-research/knowledge-center/protein-purification-methods/How-buffer-pH-and-NaCl-affect-size-exclusion-chromatography
... the mobile phase was lower or higher than the pI values of the proteins. ... The addition of acetonitrile to the buffer for the separation of lysozyme ...
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44 influence of lysozyme on egg white quality
https://etd.ohiolink.edu/apexprod/rws_etd/send_file/send?accession=osu1486561555618501&disposition=inline
^The isoelectric point of ovomucin has not been well established. ... Lysozyme values were reported as the percent of egg white.
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45 LYSOZYME, AN ABOMASAL ENZYME IN THE RUMINANTS
https://hal.archives-ouvertes.fr/hal-00901460/document
weights, but this was not significant. Five molecular forms of bovine lysozyme were revealed by electrofocusing with pi values of 6.2,.
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46 Measuring Low Concentration in Protein Lysozyme
https://www.news-medical.net/whitepaper/20190516/Measuring-Low-Concentration-in-Protein-Lysozyme.aspx
Figure 6. 1.0 mg/mL lysozyme result. For clarification: Mean Diam = 3.8 nm, Std Dev = 2.404 nm, PI = 0.399. The presence of ...
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47 CaCO3 thin-film formation mediated by a synthetic protein ...
https://pubs.rsc.org/en/content/articlepdf/2017/ra/c6ra28808a
various ratios of GG1234 to lysozyme without inducing simple coacervation. ... It has a theoretical pI value of 3.58, effi-.
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48 isolation of avidin and lysozyme from egg albumen.
https://open.library.ubc.ca/media/stream/pdf/831/1.0097373/2
of protein - protein interaction between avidin and lysozyme ... either by binding to ion exchange resins at their pi, or by.
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49 Examination of egg white proteins and effects of high pressure ...
https://digitalcommons.unl.edu/cgi/viewcontent.cgi?article=1006&context=foodscidiss
ovalbumin, ovotransferrin, and lysozyme compared to heat-treated (85 to 95°C) and ... This pH range was chosen as most pI values of major egg white.
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50 Effect of pH on Diclofenac–Lysozyme Interaction - Frontiers
https://www.frontiersin.org/articles/10.3389/fmolb.2022.872905/full
However, our finding leads us to speculate that the pI value of lysozyme and DCF are 11 and 4, respectively. Therefore, at pH 7.0, ...
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51 Heteroprotein complex formation of bovine serum albumin ...
https://www.alice.cnptia.embrapa.br/bitstream/doc/1090911/1/1s2.0S0268005X17307002main.pdf
between bovine serum albumin and lysozyme and to characterize ... 3b shows the pI values of the isolated proteins and all other ratios studied.
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52 Stomach lysozymes of ruminants. II. Amino acid sequence of ...
https://www.jbc.org/article/S0021-9258(18)90908-7/pdf
AMINO ACID SEQUENCE OF COW LYSOZYME 2 AND IMMUNOLOGICAL COMPARISONS ... Our calculated PI value of 7.65 k 0.22 agrees with the value of 7.5.
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53 Effect of Protein Aggregation on the Binding of Lysozyme to ...
https://people.chem.umass.edu/dubinlab/refs/la980366d.pdf
Lysozyme, a basic protein with a high isoelectric point of 11.5, is known to exist ... on lysozyme amino acid pK values was determined using.
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54 Isolation of Lysozyme from Chicken Egg White Using Cation ...
https://www.academia.edu/42900383/Isolation_of_Lysozyme_from_Chicken_Egg_White_Using_Cation_Exchange_Chromatography
Hen egg white lysozyme is a protein with high isoelectric point (pI=11) in ... until wells showing highest absorbance values reached a value above 1.
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55 CHARACTERIZATION OF A SALIVARY LYSOZYME IN ...
https://search.proquest.com/openview/3c4126011fad6e74a855d5298ede8526/1.pdf?pq-origsite=gscholar&cbl=54017
protein is about 14.4 kDa without the signal peptide. The pI value is greater than. 9.5 as determined by isoelectric focusing. From genomic DNA, two introns.
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56 chapter 4 - exploring proteins
https://uh.edu/sibs/faculty/cstath/Chapter4_1.pdf
their pI values. figure 5-20. Estimating the molecular weight of a protein. The elec- trophoretic mobility of a protein on an SDS polyacryl-.
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57 Partial Purification of Hen Egg White Lysozyme by Ethanol ...
https://dergipark.org.tr/tr/download/article-file/119836
The ranges of thermal inactivation parameters, such as D. (D80 °C = 29.2-59 min, D90 °C = 8.8-21 min) and z (z80-90 °C = 17.4-22.3 °C) values of the enzyme, ...
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58 Structural differences between native Hen egg white lysozyme ...
https://ui.adsabs.harvard.edu/abs/2013AcSpA.114..368B/abstract
The difference in molecular structure of native HEWL and its fibrils, grown at a pH value near physiological pH 7.4 and at a pH value just above the pI, ...
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59 A new lysozyme from the eastern oyster, Crassostrea virginica ...
https://bmcecolevol.biomedcentral.com/articles/10.1186/1471-2148-10-213
The chemical properties of cv-lysozyme 3 (including molecular weight, isoelectric point, basic amino acid residue number, and predicted protease ...
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60 Analysis and Comparison of Proteomic Profiles of Tear Fluid ...
https://iovs.arvojournals.org/article.aspx?articleid=2187689
Lysozyme has been found in the tear fluids of several domestic animals (sheep, ... digestive lysozymes tend to have a low pH optimum and low pI value, and a ...
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61 ProtParam tool - Expasy
https://web.expasy.org/protparam/
The computed parameters include the molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficient, ...
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62 To isolate and purify ovalbumin from egg white proteins by ...
https://www.numerade.com/ask/question/principles-of-protein-purification-and-quantification-aim-to-isolate-and-purify-ovalbumin-from-egg-white-proteins-by-selective-precipitation-in-combination-with-isoelectric-pointisolation-of-49447/
Recover the supernatant, which should now contain the eluted lysozyme. Why is lysozyme eluted at this stage? Group of answer choices 01.Its pI = pH here and it ...
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63 Influence of Hydrogen Ion Concentrations on the Protein ...
https://austinpublishinggroup.com/chromatography/fulltext/chromatography-v7-id1053.php
Lysozyme and Bovine Serum Albumin (BSA) fraction-V were purchased from Hi-Media, haemoglobin from ox red blood cells (RBC) and haemolymph of Bombyx mori (fifth ...
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64 Multiple forms of medicinal leech destabilase-lysozyme
http://www.rtcb.iitp.ru/PDF/12788107.pdf
terised by the amino acid variations in 64 (T or R) and 67 (H or R) positions; P-Ac, isoform of destabilase-lysozyme of calculated acid pI value.
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65 (IUCr) Orthorhombic lysozyme crystallization at acidic pH ...
https://journals.iucr.org/d/issues/2018/05/00/nj5276/
Orthorhombic lysozyme crystallization at acidic pH values driven by phosphate binding ... The structure of orthorhombic lysozyme has been obtained at 298 K and pH ...
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66 A lysozyme and magnetic bead based method of separating ...
https://go.gale.com/ps/i.do?id=GALE%7CA395420918&sid=googleScholar&v=2.1&it=r&linkaccess=abs&issn=16182642&p=AONE&sw=w
Keywords Bacteria separation * Magnetic beads * Lysozyme mutation * Protein ... Due to the high pI value (11) of chicken c-type lysozymes, this protein is ...
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67 hen-egg white lysozyme: Topics by Science.gov
https://www.science.gov/topicpages/h/hen-egg+white+lysozyme
The apparent K(M) values for hen egg white lysozyme increased with the enzyme ... 11, 15, and 20 wk post infection (PI) and in egg yolk at 11 and 20 wk PI.
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68 A Lysozyme and Magnetic Beads Based Method for ...
https://d-nb.info/1044956097/34
In its native form, lysozyme causes bacteriolysis due to the catalytic amino acid ... The hen egg white lysozyme has a high isoelectric point (pI) value.
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69 Ortho-methylated 3-hydroxypyridines hinder hen egg-white ...
https://www.nature.com/articles/srep12052
Lysozyme is the protein involved in the latter and it is widely ... and 4HP prolonged the fibrillization lag-phase and reduced the kf value.
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70 isoelectric point and ph - The People's National Party
https://pnp.org.jm/la-fortune/isoelectric-point-and-ph
Proteins such as lysozyme, cytochrome c, histone, If the pH of the ... The isoelectric point (pI) is the pH value at which the molecule ...
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71 Protein Phase Behavior in Aqueous Solutions: Crystallization ...
https://www.cell.com/fulltext/S0006-3495(08)70734-3
The lysozyme crystal solubility in sodium chloride at pH 7 is also compared to the corresponding b2 values in Fig. 12 D. Figure thumbnail gr12. Figure 12 ...
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72 Experiment 2: Purification of Lysozyme from Egg White Part 1
https://www.studocu.com/en-ca/document/university-of-winnipeg/introduction-to-biochemistry/experiment-3-report-guide/16285039
Lysozyme is the protein that was eluted in Fraction E when the 100mM of carbonate buffer was added because it has an pI of 10 as shown in Table 3.
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73 Purification of Egg White Lysozyme from Indonesian Kampung ...
https://journal.ipb.ac.id/index.php/mediapeternakan/article/view/8992/7272
Given weight of eggs of kampung chicken is significantly lower to that of Cihateup duck egg and commercial laying hens, the HU value of kampung.
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74 According to the results in table 8 the percentage of lysozyme ...
https://www.coursehero.com/file/p7amvvi/According-to-the-results-in-table-8-the-percentage-of-lysozyme-A-B-C-and-D-in/
Lysozyme D shows the highest purity in this experiment. However, the theoretical resultof lysozyme D is 3.5%. This deviation can be attributed to experimental ...
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75 Charge Variant Analysis - Agilent
https://www.agilent.com/cs/library/applications/compendium-chargevariant-advancebio-5994-0034EN-us-agilent.pdf
point, pI, is the pH at which the net charge of the protein is neutral (the ... If the pH is below this value, the protein will ... Lysozyme, pI = 11.0.
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76 Chapter 11 Two Reliable and Inexpensive Lysozyme Assays ...
https://www.ableweb.org/biologylabs/wp-content/uploads/volumes/vol-3/11-snyder.pdf
Lysozyme Assays. Introduction. A simple and reliable enzyme assay is a valuable teaching aid in an in- troductory biology course. Of even greater value is ...
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77 Affinity purification of hen egg lysozyme using sephadex G75
https://www.ajol.info/index.php/ajb/article/view/55898/44356
Key words: Lysozyme purification, sephadex G-75, hen eggs. INTRODUCTION ... binding at pH 6.0, suggest a charge group with pKa value.
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78 Alpha_Lactalbumin
https://collab.its.virginia.edu/access/content/group/61ef6ea0-869d-476a-990a-9738e3899823/1/06__DoV__AlphaLactalbumin-Homo%20sapiens__1A4V/06__DoV__AlphaLactalbumin-Homo%20sapiens__1A4V_Alpha_Lactalbumin.html
Alpha-Lactalbumin (1A4V) from Homo Sapiens is a small and acidic protein as evidenced by its molecular weight (14,200 kDa) [10] and isoelectric point (pI= ...
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79 Isolation and Purification of Lysozyme from Egg Whites
https://www.wcmo.edu/academics/signature/usf/files/Carey,-LaFlamme,-Needy.pdf
The mechanism of Micrococcus lysodeikticus cell lysing with a lysozyme ... Record the absorbance value in 15 second intervals for the first 120 seconds,.
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80 Dry-Heating Makes Hen Egg White Lysozyme an Efficient ...
https://perso.univ-rennes1.fr/franck.artzner/publi/36_desfougeres_AgFchem.pdf
However, the experiments were not performed at pH 10.7, the isoelectric point of lysozyme, because it was a pH value too high to be sure that.
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81 Functional Proteins and Peptides of Hen's Egg Origin
https://cdn.intechopen.com/pdfs/42431/InTech-Functional_proteins_and_peptides_of_hen_s_egg_origin.pdf
In addition to excellent nutritional value, egg proteins have unique ... Proteins in the egg white as lysozyme, ... and an isoelectric point of 4.5.
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82 Partial Purification of Hen Egg White Lysozyme by Ethanol ...
https://aj.tubitak.gov.tr/agriculture/issues/tar-07-31-2/tar-31-2-6-0701-5.pdf
The ranges of thermal inactivation parameters, such as D. (D80 °C = 29.2-59 min, D90 °C = 8.8-21 min) and z (z80-90 °C = 17.4-22.3 °C) values of the enzyme, ...
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83 RAFT polymers for protein recognition - Beilstein Journals
https://www.beilstein-journals.org/bjoc/articles/6/66
With respect to varying pI values, both RAFT polymers display little selectivity: From lysozyme (pI > 9) down to BSA (pI < 6) protein affinities vary by ...
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84 Chpt. 5 Protein Isolation Flashcards | Quizlet
https://quizlet.com/230930377/chpt-5-protein-isolation-flash-cards/
Protein A, isoelectric point (pI)=4.1, Mass=80 kD Protein B, pI=9.0, Mass=81 kD Protein C, pI=8.8, Mass=37 kD Protein D, pI=3.9, Mass=172 kD How do you separate ...
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85 Purification and Characterization of Lysozyme Produced by ...
https://www.jstage.jst.go.jp/article/biochemistry1922/83/3/83_3_893/_pdf
by Streptomyces erythraeus; Ch lysozyme, lysozyme ... lysozyme was determined to be. pI ... value of. Mw. (18,500) was ob tained by extrapolation.
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86 A mixture of three proteins (A) (pepsin), (B) (haemoglobin) and ...
https://www.toppr.com/ask/question/a-mixture-of-three-proteins-a-pepsin-b-haemoglobin-and/
The pH at isoelectric point (pI) of the proteins are pI of (A), (B) and (C) which are ... and (C) (lysozyme) was separated by eletrophoresis method at pH=7.
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87 Calculating Isoelectric Point of Proteins - AK Lectures
https://aklectures.com/lecture/purification-and-sequencing-of-proteins/calculating-isoelectric-point-of-proteins
In order to determine the isoelectric point a given protein, we must follow a general rule that consists of two steps (1) Estimate the pH value at which ...
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88 Major egg white allergens
https://clinmedjournals.org/articles/ijam/ijam-1-009table1.html
Ovotransferin, Gal d 3, 13, 22-94, 77.7 ; Lysozyme, Gal d 4, 3.5, 6-69, 14.3 ; pI: isoelectric point.
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89 Lysozyme Experiment Manual | St. Olaf Pages
https://pages.stolaf.edu/wp-content/uploads/sites/803/2016/12/Carney_Giannini_Lysozyme_Experiment_Manual_20161222.pdf
Lysozyme is an excellent protein for studying enzyme activity. ... the R2 value is to 1.0, the better the fit of the regression line.
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90 Calculating Net Charge on Proteins - YouTube
https://www.youtube.com/watch?v=g0TZncwkJxU
Feb 4, 2015
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91 Calculating Isoelectric Point of Proteins (Example) - YouTube
https://www.youtube.com/watch?v=c2kK4My2Jzc
Feb 4, 2015
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92 13.1: Amino Acids - Chemistry LibreTexts
https://chem.libretexts.org/Bookshelves/Introductory_Chemistry/Book%3A_Chemistry_for_Allied_Health_(Soult)/13%3A_Amino_Acids_and_Proteins/13.01%3A_Amino_Acids
Each amino acid has its own pI value based on the properties of the amino acid. At pH values above or below the isoelectric point, the molecule ...
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93 Food Macromolecules and Colloids - Page 242 - Google Books Result
https://books.google.com/books?id=8GwoDwAAQBAJ&pg=PA242&lpg=PA242&dq=lysozyme+pi+value&source=bl&ots=XzKGFoq9z4&sig=ACfU3U16rtPyw3MP2xovnl6OSoZxTs8GbQ&hl=en&sa=X&ved=2ahUKEwicxdngz8_7AhUvUKQEHcUaB9IQ6AF6BQjgAhAD
... protein (BSA or lysozyme) within the inner droplets of multiple emulsions is ... The yield of the multiple emulsion and the pi value of the encapsulated ...
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94 Handbook of eggs in human function - Page 41 - Google Books Result
https://books.google.com/books?id=UBPTDwAAQBAJ&pg=PA41&lpg=PA41&dq=lysozyme+pi+value&source=bl&ots=vKOUYNcdLq&sig=ACfU3U2mPshSam6W5lTKjXf0p2Z7hr2Ctw&hl=en&sa=X&ved=2ahUKEwicxdngz8_7AhUvUKQEHcUaB9IQ6AF6BQjUAhAD
Lysozyme is among the first egg white proteins that has been isolated and used by ... lysozyme using ion exchange chromatography is due to its high PI value ...
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95 Protein-Protein Interactions: Computational and Experimental ...
https://books.google.com/books?id=ytqgDwAAQBAJ&pg=PA361&lpg=PA361&dq=lysozyme+pi+value&source=bl&ots=b82jAT0Mep&sig=ACfU3U2AoWFkVNnKIqoigHkphleLzzZGSg&hl=en&sa=X&ved=2ahUKEwicxdngz8_7AhUvUKQEHcUaB9IQ6AF6BQjiAhAD
Ducruix in solubility measurement of lysozyme in salt solutions at pH below its pI where the protein ... kilo-Dalton (kD) with a high pI value of ∼11(12).
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