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1 How do you mix PCR reactions - swish, flick or nothing?
http://www.protocol-online.org/biology-forums/posts/5818more1.html
In the olden days, the theory was that you should never vortex enzymes since it was thought that would shear them.
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2 beginner lab question- what is ok to vortex / thaw quickly?
https://www.reddit.com/r/biology/comments/waqkb/beginner_lab_question_what_is_ok_to_vortex_thaw/
Enzymes typically do not like to be vortexed, nor should they go through freeze thaws. However, some enzymes are more robust than others but in general try not ...
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3 Is it bad to mix a test tube, containing enzymes and substrates ...
https://www.quora.com/Is-it-bad-to-mix-a-test-tube-containing-enzymes-and-substrates-using-a-vortex-mixer-if-you-want-to-test-the-enzyme-activity-on-the-substrates
Yes, it is bad because vortex mixers induce bubbles and foaming, which in turn will cause the protein to denature and you will get variable enzyme activity ...
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4 Do someone have an observation about how enzymatic ...
https://www.researchgate.net/post/Do_someone_have_an_observation_about_how_enzymatic_activity_of_RNase_depends_of_vortexing
Shear stress can damage the enzyme. It's better to mix gently with pipette. ... Usually the Syber premix contains detergents and if you vortex then the resultant ...
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5 Vortex-mixing-induced inactivation of arginine-specific ADP ...
https://pubmed.ncbi.nlm.nih.gov/8019426/
The vortex-mixing-induced inactivation of the diluted enzyme was rapid; more than 85% of the enzyme activity was lost with 1-min vortex-mixing at room ...
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6 Accurate Enzymatic Assays in PCR Tubes - Lab On The Cheap!
https://www.labonthecheap.com/accurate-enzymatic-assays-in-pcr-tubes/
Considerable time can be saved by the second option, the 96-well plate method, and robust enzymes will react willingly without a thorough vortex ...
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7 Is it bad to mix a test tube containing enzymes and substrates ...
https://homework.study.com/explanation/is-it-bad-to-mix-a-test-tube-containing-enzymes-and-substrates-using-a-vortex-mixer-if-you-want-to-test-the-enzyme-activity-on-the-substrates.html
The vortexing isn't recommended since it could disrupt the three-dimensional structure of the enzyme and hamper its activity.
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8 Can you vortex enzymes? - News Share
https://www.newsshare.in/can-you-vortex-enzymes-116609.html
Can I Vortex Taq? Do not vortex PCR mix. Add DNA polymerase (Taq) to the reaction tube last. … Avoid overloading PCR products into the gel; this ...
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9 FAQ: How should I set up a restriction digest? - NEB
https://www.neb.com/faqs/2012/09/13/how-should-i-set-up-a-restriction-digest
The reaction must be thoroughly mixed to achieve complete digestion. We recommend gently pipetting ther reaction mixture up and down or " ...
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10 Mischief in the Mastermix: Bothersome Bubbles - Bitesize Bio
https://bitesizebio.com/20757/mischief-in-the-mastermix-bothersome-bubbles/
If you have a table mini-centrifuge, let the solution spin for a couple of seconds. Alternatively, you can try vortexing the mix on low-medium ...
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11 Restriction Enzyme Digest Troubleshoot | ZAGENO
https://zageno.com/l/ts-restriction-enzyme-digest
Do not vortex the restriction enzyme, just mix by flicking or pipetting up and down. Perform a digestion reaction with a standard control DNA, e.g. Lambda DNA.
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12 BglII, Restriction Enzymes - Jena Bioscience
https://www.jenabioscience.com/molecular-biology/enzymes/restriction-enzymes/b-enzymes/en-106-bglii
EN-106 / Enzymes Restriction Enzymes "B" Enzymes. ... Unit Definition: One unit is the amount of enzyme required to completely digest 1 ... Do not vortex.
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13 A pretend biologist's guide to running a PCR (polymerase ...
https://samnicholls.net/2016/06/21/pcr-protocol/
A highly thermostable polymerase enzyme (a molecular machine for assembling ... Vortex and centrifuge the dNTP mix briefly if you have not ...
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14 Vortex fluidics-mediated DNA rescue from formalin-fixed ...
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225807
To reduce processing time and improve rescue efficiency, we applied the mechanical energy of a vortex fluidic device (VFD) to drive the ...
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15 Vortex mixer - Wikipedia
https://en.wikipedia.org/wiki/Vortex_mixer
A vortex mixer, or vortexer, is a simple device used commonly in laboratories to mix small vials of liquid. It consists of an electric motor with the drive ...
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16 Restriction Enzyme Resource Guide - Promega Corporation
https://www.promega.com/resources/guides/nucleic-acid-analysis/restriction-enzyme-resource/
Some of these cookies are essential for our website to work. For others, we won't set them unless you accept them. To find out more about cookies and how to ...
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17 Vortex Mixers - Conduct Science
https://conductscience.com/specimen-lab/vortex-mixers/
First, place the sample of interest, for example, a test tube, into the rubber piece so that it will also revolve in a circular motion. Most vortex mixers ...
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18 Accelerating Enzymatic Catalysis Using Vortex Fluidics - 2016
https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201604014?_utm_campaign=mention57529&_utm_medium=inline&_utm_content=lnk241316896300&_utm_source=xakep
Each enzyme responds best to specific frequencies of pressure waves, and an acceleration landscape for each protein is reported. A vortex ...
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19 The Benefits of a Vortex Mixer - AZoM
https://www.azom.com/article.aspx?ArticleID=17282
Vortex mixers are one of the primary technologies for mixing laboratory samples in test tubes, flasks, or well plates which use a simple mechanism to ...
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20 Plasmid DNA - Protocols · Benchling
https://benchling.com/protocols/lxhyWKdp/diagnostic-restriction-digestion-drd/sbs
The last thing to do, since we don't want to destroy the enzymes: Get restriction enzymes from the freezer (keep on ice or in the blue frozen holder thing), ...
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21 How to Work with Enzymes Supplied as Ammonium Sulfate ...
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/protein-biology/enzyme-activity-assays/ammonium-sulfate-suspensions
Make sure the cap is on tight, and then invert the vial/bottle several times to provide a milky suspension. This may take a minute or so. Do not vortex or ...
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22 DNase Max® Handbook - Labettor
https://labettor.com/uploads/products/protocols/5503.pdf
www.qiagen.com/safety where you can find, view and print the SDS for each QIAGEN ... Do not vortex the DNase I. It will denature the enzyme and decrease its ...
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23 Vortex Mixers | Next Day Science
https://www.nextdayscience.com/vortex-mixers/
Literally, if you need to mix oil and water, a vortex mixer is the equipment you want to get the job done right. In addition, if you want to dilute an acid or ...
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24 Troubleshooting – Restriction Enzymes | NZYTech
https://www.nzytech.com/wp-content/uploads/2019/12/conventionalnzyrestrictionenzymes_troubleshooting_v1901.pdf
Minimize exposure of enzyme to temperatures above -20°C and avoid vortex samples or reactions containing restriction enzymes. Check respective expiry date. If ...
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25 Vortex Mixers | Thermo Fisher Scientific
https://www.thermofisher.com/search/browse/category/us/en/90179108/Vortex%20Mixers
The Thermo Scientific™ Basic Vortex Mixer provides comprehensive mixing capabilities for enzymatic and RIA assays, general test tube mixing, biochemical assays, ...
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26 an awesome lab trick! Finger vortexing & other mixing methods
https://www.youtube.com/watch?v=cF0qvsV6OD8
the bumbling biochemist
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27 Accelerating Enzymatic Catalysis Using Vortex Fluidics.
https://europepmc.org/article/med/27493015
Each enzyme responds best to specific frequencies of pressure waves, and an acceleration landscape for each protein is reported. A vortex fluidic device ...
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28 Simulate Restriction Cloning - SnapGene
https://www.snapgene.com/guides/simulate-restriction-cloning
With the vector, ensure that the digestion and phosphatase reactions go to completion. Vortex the mixtures thoroughly and repeatedly, and do not allow any ...
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29 Vortex Mixers | Fisher Scientific
https://www.fishersci.com/us/en/browse/90179108/vortex-mixers?categoryKey=90179108&page=2
The variable speed shaking allows you to adjust the level of mixing from gentle shaking to vigorous vortexing. Easy-to-change platforms allow you to adapt your ...
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30 Can I vortex my antibody or recombinant protein? - Omnimabs®
http://www.omnimabs.com/support/detail-c-3.html?o=1
Yes, you can but keep it short (pulse vortex), as it is always best to maximize the health of your cells. · Can I vortex samples that have been stained with ...
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31 manual Gibson Assembly Master Mix E2611
https://www.med.unc.edu/pharm/sondeklab/wp-content/uploads/sites/868/2019/10/gibson-cloning.pdf
Before use, thaw and vortex the master mix thoroughly and keep on ice. ... sequence can be composed of nucleotides which belong to only one fragment.
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32 ExiSpin from Bioneer
https://us.bioneer.com/products/instrument/ExiSpinoverview.aspx
Once you try the ExiSpin, you will find it an essential instrument for you ... Full speed vortex and centrifuge: No need to vortex by hand and load a ...
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33 QIAGEN Multiplex PCR Kit
https://www.qiagen.com/us/products/discovery-and-translational-research/pcr-qpcr-dpcr/pcr-enzymes-and-kits/end-point-pcr/qiagen-multiplex-pcr-kit/
The kit can be successfully used for various multiplex applications such as typing ... AII (part A) and the indicated amounts of enzyme per 50 µl reaction.
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34 Boiling RNA Preparation: for RNA isolation (from cell pellets).
https://www.science.smith.edu/cmbs/wp-content/uploads/sites/36/2015/09/CWZ-Mini-Boiling-RNA-Preparation-BCH-390-version.pdf
Never vortex or warm enzymes – they are too unstable! You can flick tube gently with finger. You want to be as gentle as possible with your ...
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35 Enzymes and reagents | LGC Biosearch Technologies
https://www.biosearchtech.com/enzymes-and-reagents-8112
You do not require a fully automated solution, but still need high throughput. ... vortex mixing to lyse the cells and prepare the RNA for cDNA synthesis.
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36 Taylor-Couette Vortex Flow in Enzymatic Reactors
https://link.springer.com/protocol/10.1007/978-1-59745-053-9_28
One important reason for using vortex flow in biochemical reactors is its ability to promote a gentle, but still efficient, stirring, which is ideal when ...
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37 Restriction Enzyme Digest of Plasmid and Insert DNA
https://www.neosynbio.com/restriction-enzyme-digest-of-plasmid-and-insert-dna
Retrieve 10x restriction buffer from freezer, thaw completely, and vortex to mix. The same tube of buffer can be used many times, if you are careful with ...
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38 PCR Sample Preparation - DNA Isolation, RNA Extraction
https://www.bosterbio.com/protocol-and-troubleshooting/pcr-sample-preparation
You can use an enzymatic method or a mechanical method. Add 1ml of cell suspension into 2 ml tubes; Add 100 mg of 0.5 mm glass beads and vortex for 15 min.
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39 Protocols for Experiment 1
https://instruct.uwo.ca/biology/3596a/Protocol1.html
It is important that you do not vortex the tube because this will shear the genomic DNA. It is important not to let the lysis reaction proceed for more than ...
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40 Frequently Asked Questions About Proteinase K - AG Scientific
https://agscientific.com/blog/proteinase-k-faqs.html
To determine if the enzyme is working, you can do the following 2 steps: Find how many micromoles of the p-nitroanilide are produced per minute. Then divide by ...
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41 Vortex Mixer- Definition, Principle, Parts, Types, Examples, Uses
https://microbenotes.com/vortex-mixer-definition-principle-parts-types-examples-uses/
A standard stirrer can only mix one sample container at a time. It cannot combine fluids in sealed flasks, and it is sometimes necessary to ...
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42 Vortex Mixer: Principle, Types, and Uses - Microbe Online
https://microbeonline.com/vortex-mixer-principle-types-and-uses/
In the analysis of proteins and enzymes: Proper mixing of samples with reagents and buffer is a critical step in studying proteins and enzymes.
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43 Taq Full DNA Polymerase User Manual - Takara Bio
https://www.takarabio.com/documents/User%20Manual/PT3820/PT3820-1.pdf
enables you to use less enzyme in each PCR reaction. Favorable results can be generated using as little as 0.2 units in a single reaction ( ...
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44 Manual: Just cDNA Double-Stranded cDNA Synthesis Kit
https://www.chem-agilent.com/pdf/strata/200453.pdf
15 U. Escherichia coli DNA polymerase I (9.0 U/μl). 500 U. Sodium acetate (3 M) ... solution. Do not vortex the enzyme stock tubes.
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45 Can you vortex taq polymerase? Explained by FAQ Blog
https://faq-blog.com/can-you-vortex-taq-polymerase
Can you vortex enzymes? ... Do not vortex enzyme reactions. You will need to physically mix the enzyme into the reaction because the enzyme exists ...
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46 Genome sequence of the pattern forming Paenibacillus vortex ...
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-11-710
In Figure 5A, we show that propagating P. vortex swarms can collectively ... Clusters of Multifunctional Enzymes-Secondary Metabolites.
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47 ONT dA-tailing for Fungal Barcoding - Protocols.io
https://www.protocols.io/view/ont-da-tailing-for-fungal-barcoding-b9qqr5vw
Important: Vortex the Ultra II End-prep Reaction Buffer for 30 seconds. (Do not vortex the End-prep Enzyme Mix). 5. In a 0.2mL thin wall, sterile, ...
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48 Should you vortex antibodies? - All Famous Faqs
https://allfamousbirthday.com/faqs/should-you-vortex-antibodies/
Should you vortex antibodies? Whenever you dilute an antibody, mix it gently to ensure a homogeneous solution. We recommend against using a ...
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49 Dos and Don'ts for molecular testing
https://www.who.int/teams/global-malaria-programme/case-management/diagnosis/nucleic-acid-amplification-based-diagnostics/dos-and-don-ts-for-molecular-testing
Do not clean the vortex and centrifuge with sodium hypochlorite; instead, ... This may help to avoid false-negative results from enzyme ...
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50 Enzymes, Laboratory Notes for BIO 1003
https://faculty.baruch.cuny.edu/jwahlert/bio1003/enzymes.html
We will be using the enzyme catecholase (or catechol oxidase) that is common ... and stir the mixture with a vortex genie or by tapping the side of the tube ...
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51 Restriction Digests - College of Biological Sciences |
https://cbs.umn.edu/springer-lab/protocols/restrictiondigests
Prepare the bulk mix on ice, adding enzyme last. Mix well (do not vortex). Aliquot bulk mix into reaction tubes. Mix by inversion (do not vortex). Incubate at ...
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52 Gibson Assembly Cloning Guide, second edition
https://www.biocat.com/bc/files/Gibson_Guide_V2_101417_web_version_8.5_x_11_FINAL.pdf
The Gibson Assembly® method can be used to rapidly clone multiple DNA fragments into any vector in one hour or less without the use of restriction enzymes.
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53 Restriction Endonucleases - New England Biolabs
https://www.neb.uk.com/neb/media/documents/RestEndo_TechGuide_0717_UK_LR.pdf
Online tutorials on how to avoid star activity and ... digests, you will benefit from the high quality of our enzymes. ... Do not vortex the reaction.
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54 Vortex Mixers and Lab Blenders - Stellar Scientific
https://www.stellarscientific.com/vortex-mixers/
A vortex mixer uses quick bursts of high-rpm energy to create a vortex effect for mixing and blending lab samples.
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55 YeaStar Genomic DNA Kit™ - Zymo Research
https://files.zymoresearch.com/protocols/_d2002__yeastar_genomic_dna_kit.pdf
restriction enzyme digestion, etc. ... Note: You can vortex hard for 10-20 seconds after adding YD Lysis Buffer. This will.
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56 plasmidlab.doc - Lab 7: Molecular Biology
https://www.bio.fsu.edu/~epstein/ysp/Lab%20Chapters/plasmidlab.doc
You will first extract plasmid DNA from bacteria. You will then digest (break apart) samples of your DNA with restriction enzymes in preparation for the ...
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57 Set Yourself up for Success with Flow Cytometry| Bio-Rad
https://www.bio-rad-antibodies.com/blog/set-yourself-up-for-success-with-flow-cytometry.html
You shouldn't vortex vigorously; try and avoid bubbles as the surface tension of the bubbles can kill cells (Walls et al. 2017).
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58 FLUOROPHORE-ASSISTED-CARBOHYDRATE ...
https://www.lerner.ccf.org/bme/hascall/documents/PEGNAC%20-%20FACE%20Protocol%20for%20GAGs%201-14-16.pdf
Add 200 µl 100 mM ammonium acetate, containing 0.1% BSA, to 0.5 U of enzyme. Vortex/Spin. Incubate at. RT 20 min. Vortex/Spin.
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59 Vortex Mixer - Definition, Principle, Parts, Uses
https://microbiologynote.com/vortex-mixer/
Mixing samples with reagents and buffer is an essential step in the analysis of proteins and enzymes. Consequently, a vortex mixer facilitates ...
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60 Optimizing Restriction Endonuclease Reactions - NEB
https://www.nebiolabs.com.au/tools-and-resources/usage-guidelines/optimizing-restriction-endonuclease-reactions
Do not vortex the reaction. In general, we recommend 5–10 units of enzyme per µg DNA, and 10–20 units for genomic DNA in a 1 hour digest.
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61 Cloning Protocol - OpenWetWare
https://openwetware.org/wiki/Cloning_Protocol
You will need to have the proper restriction enzymes picked out so that they cut the plasmid ... Vortex them and put them back in the ice for 2-30 minutes.
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62 Restriction enzymes and PCR - iGEM
https://static.igem.org/mediawiki/2013/6/66/RestrictionEnzymes_and_PCR.pdf
Enzymes or enzymatic ... Vortex or finger-flick reagents to mix well before use ... If you're intending to fuse protein domains, BE CAREFUL. Frame.
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63 Restriction Digest and agarose gel electrophoresis
https://joannecpratt.com/courses/principles-of-modern-biology/laboratory-procedures/week-6-restriction-digest-and-agarose-gel-electrophoresis/
While they are incubating, you can prepare aliquots of your PCR reactions for ... “uncut” = a control, run in parallel, without restriction enzymes added; ...
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64 Part D: Protocol PCR – BBS OER Lab Manual
https://ecampusontario.pressbooks.pub/biochem2l06/chapter/1-2-3-protocol-part-b-pcr/
You will use the PCR primers you designed in one reaction, and the PCR ... Once added, vortex the tubes for 1-2 minutes (make sure you fasten the cap ...
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65 20 Answers to Important Proteinase K Questions - GoldBio
https://goldbio.com/articles/article/20-answers-to-important-proteinase-k-questions-plus-free-printable-fact-sheet
How do you inactivate proteinase K? Inactivating proteinase K is ... Keep in mind, however, that heating proteinase K does not fully inactivate the enzyme.
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66 In Vitro Transcription from Plasmid or PCR-amplified DNA
https://www.sciencedirect.com/science/article/pii/B9780124200371000051
It will fall out of solution when added to the mix, so vortex it to resuspend before adding enzyme. 6.8. Tip. A successful transcription usually yields a white ...
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67 Molecular Cloning - New England Biolabs GmbH
https://www.neb-online.de/wp-content/uploads/2018/05/MolCloning_Tech_Guide_D-F_1805_LowRes.pdf
Do not vortex the reaction. • In general, we recommend 5 – 10 units of enzyme per µg DNA, and 10 – 20 units per µg of genomic DNA in a 1 hour digest.
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68 Restriction Digest
https://www.cwcboe.org/cms/lib/NJ01001185/Centricity/Domain/143/Molecular%20Biology/Restriction%20digest%20and%20Gel/DNA_restriction_digest.pdf
When more than one restriction endonuclease is used in a ... Restriction enzymes can be used to determine the ... Add the enzyme last just in case you.
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69 Yeast Cell Disruption Methods - OPS Diagnostics
https://opsdiagnostics.com/applications/applicationtable/microorganism/yeast.htm
Bead beating can be performed using a Pulsing Vortex Mixer, HT Mini homogenizer, GenoGrinder, or HT Homogenizer. Unfortunately, vortexing samples mixed with ...
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70 Multi-V-32 - Vortex mixer for intensive stirring
https://www.labortechnik.com/en/vortex-mixer-multi-v32/mini-vortex-strong
Multi-Vortex V-32 is intended for intensive stirring of bacterial and yeast cell, washing from the culture medium and extraction of metabolites and enzymes ...
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71 Preparing Whole Cell Protein Extracts – Basic Protocol
https://www.uthsc.edu/research/institutional-cores/pmc/documents/03-preparing-whole-cell-protein-extracts-for-differential-expession-analysis.docx
Daniel Johnson in the Molecular Bioinformatics (mBIO) core (x8-3743) if you are unsure about statistical requirements for an experiment. Cell Lysis and Protein ...
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72 BIOSAN V-32 Multi-Vortex - GeneCraft Labs
https://genecraftlabs.com/en/product/biosan-v-32-multi-vortex/
BIOSAN V-32 Multi-Vortex is intended for an intensive stirring of bacterial and ... and extraction of metabolites and enzymes from cells and cell cultures.
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73 KAPA Frag Kit for Enzymatic Fragmentation
https://rochesequencingstore.com/wp-content/uploads/2018/01/KAPA-Frag-Kit.pdf
and a detailed protocol for the KAPA Frag Kit for Enzymatic ... guidelines on how to systematically optimize the ... Vortex gently and proceed.
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74 Frequently asked questions | Support - TwistDx
https://www.twistdx.co.uk/support/faqs/
Can I add the components of the rehydration solution one at a time directly to the freeze dried reaction pellets?
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75 Droplet Digital™ PCR Applications Guide - Bio-Rad
https://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6407.pdf
ddPCR is an end-point measurement that enables you to quantify nucleic acids ... If added at a relatively high concentration, some restriction enzyme ...
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76 Best Practices for Preparing a Single Cell Suspension from ...
https://wp.unil.ch/fcf/files/2019/03/Reichard_et_al-2019-Cytometry_Part_A.pdf
One common glycoprotein found in the extracellular matrix is ... teins can be cleaved by the proteolytic activity of enzymes.
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77 The Importance of Tissue Dissociation Enzymes in Cell ...
https://levitasbio.com/blog-the-importance-of-tissue-dissociation-enzymes-in-cell-tissue-culture/
These types of analyses can help one understand the relationship of ... in which using a pipette (for trituration) or a vortex can help ...
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78 Molecular cloning of PCR products: Restriction digestion guide
https://blog.quartzy.com/2017/02/14/molecular-cloning-pcr-products-restriction-digestion
A vector digested with only one enzyme will need to be de-phosphorylated to prevent self-ligation. A vector that ligates upon itself will ...
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79 Microbial enzymes induce colitis by reactivating triclosan in ...
https://www.nature.com/articles/s41467-021-27762-y
First, we cultured gut bacteria under anaerobic conditions and tested whether the cultured bacteria can convert TCS-G to TCS in vitro. We found ...
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80 Complex I Enzyme Activity Microplate Assay Kit (ab109721)
https://www.abcam.com/complex-i-enzyme-activity-microplate-assay-kit-colorimetric-ab109721.html
Rehydrate 100X Dye by adding 0.25 mL H2O, then vortex each thoroughly until ... enzymes, mitochondrial function, and oxidative stress, and also how to assay ...
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81 Beta-Galactosidase Activity Assay - Roth Lab
http://rothlab.ucdavis.edu/protocols/beta-galactosidase-3.shtml
For most activities, 0.5 mL cells + 0.5 mL Z buffer will produce a ... sources in the growth medium on the β-galactosidase enzyme activity.
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82 Regulation of Gene Expression: Turning Genes On and Off
http://www.ableweb.org/volumes/vol-31/?art=7
You will observe how the activity of this enzyme changes upon the addition of ... tubes on the vortex and return them to the ice bath.
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83 PCR | KOD FX | TOYOBO Biotech support Department
https://www.toyobo-global.com/seihin/xr/lifescience/products/pcr_002.html
KOD FX enzyme solution contains two types of anti-KOD DNA polymerase ... One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTP ...
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84 PAPAIN-CLARASE
https://cmbe.engr.uga.edu/bche4510/assign/Handouts/Ch%203%20A3%20papain%20specs%20CBS.pdf
PAPAIN-CLARASE is the commercial name of a mixture of proteolytic enzymes ... One NF tyrosine unit is the activity which will liberate the equivalent of one ...
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85 Enzymes
https://gbcnv.instructure.com/files/57378567/download?download_frd=1
beaker/bottle with 1% catechol solution, and vortex mixer (optional). Experiment 2- Enzyme ... In this lab you will be able to see how these enzymes work.
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86 MUMPS virus standard RT-PCR overview - CDC
https://www.cdc.gov/mumps/downloads/mumps-standard-genotyping-RT-PCR.pdf
This RT-PCR assay can be used to detect mumps RNA in clinical samples or in infected cell ... Thaw all kit reagents except enzymes, vortex and place on ice.
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87 A Cytoskeletal Vortex Drives Phage Nucleus Rotation During ...
https://www.biorxiv.org/content/10.1101/2021.10.25.465362.full
Using fluorescent protein fusions, we found that the putative Goslar-encoded DNA repair enzyme UvsX (gp193) localizes with the Goslar phage ...
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88 ChIP-IT® Express Enzymatic - Active Motif
https://www.activemotif.com/documents/1719.pdf
If you need to shear more chromatin, the stand-alone ChIP-IT Express Shearing Kit provides just the reagents to perform and validate the shearing portion of ...
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89 RK20202 StepWise DNA Lib Prep Kit Protocol - INTERCHIM
https://www.interchim.fr/ft/B/B2YI90.pdf
reagents are one year when stored properly. Mechanical or enzymatic ... Do not vortex enzymes, mix components by gently pipetting up and down several times.
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90 ddRADseq protocol | McDaniel Lab
https://mcdaniellab.biology.ufl.edu/wp-content/uploads/sites/37/ddRad_seq_protocol_McDaniel_lab-150611.pdf
restriction enzyme selection and adaptor design, which we do not discuss here. We assume ... Seal samples, vortex, centrifuge, and incubate.
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91 Vortex - Temporary Percutaneous, Transvalvular Circulatory ...
https://clinicaltrials.gov/ct2/show/NCT04674111
The Vortex System is indicated to provide temporary circulatory support in ... (Childs Class C) with elevation of liver enzymes and bilirubin > 3× upper ...
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92 Gibson Assembly® HiFi kit — User guide - Codex DNA
https://files.codexdna.com/docs/43003-Gibson-Assembly-HiFi-User-Guide.pdf
Vortex mixer. Additional optional materials. The following items are only needed if you are generating. DNA fragments with PCR.
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93 Functional profiling of circulating tumor cells with an ... - PNAS
https://www.pnas.org/doi/abs/10.1073/pnas.1803884115
We reveal an intriguing correlation between CTC protease activity and ... Whether circulating tumor cells (CTCs) secrete these enzymes to ...
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94 Restriction enzyme accessibility assay - Bio-protocol
https://bio-protocol.org/prep725
How to cite. nfavorite Favorite. Restriction Enzyme Accessibility (REA) assays are used to evaluate for chromatin remodeling activity in ...
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95 Enzyme Activity
https://www.austincc.edu/sziser/Bio1724/2402%20Labs/2402%20Lab%20-%20Enzymes.pdf
We will study some of the simple enzymes ... Incubate at the appropriate temperature for 1 hr, shaking tubes intermittently using the vortex mixer.
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96 Genetics Lab Exam #1 Review (Labs 5-9) Flashcards | Quizlet
https://quizlet.com/536994089/genetics-lab-exam-1-review-labs-5-9-flash-cards/
how to determine the number of cuts in the human genome for each enzyme? ... flick the tube; DO NOT vortex to mix as this can shear the DNA into small ...
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97 gBlocks and gBlocks HiFi Gene Fragments | IDT
https://www.idtdna.com/pages/products/genes-and-gene-fragments/double-stranded-dna-fragments/gblocks-gene-fragments
The pellets can stick to the side or cap of the tube, and unless you vortex vigorously and incubate the tube at 50°C for 20 minutes, you might not have the DNA ...
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